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UK4

Metabarcoding validation framework 2026/2027

  • First published: 28 July 2026
  • Last modified: 28 July 2026
  • Version: N/A
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Contents

Summary

OCID:
ocds-h6vhtk-06d396
Published by:
Natural England
Authority ID:
AA21675
Publication date:
28 July 2026
Deadline date:
17 August 2026
Notice type:
UK4
Has documents:
No
Has SPD:
No
Has Carbon Reduction Plan:
N/A

Abstract

DNA-based methods have the potential to significantly change how we monitor and assess ecosystems. Natural England has been exploring the use of these methods for environmental monitoring for several years, delivering a series of reports which focus on the development of DNA-based methods with potential in a particular area. These methods are now being used more widely within Natural England, particularly the detection of ecological communities using environmental DNA (eDNA). This is often conducted by employing metabarcoding assays which target specific species assemblages (e.g. fish, mammals) or broader taxonomic groups (e.g. vertebrates, invertebrates, bacteria).The DNA Team aims to operationalise DNA-based methods and produces guidance for using them in monitoring projects. In order to provide sound recommendations, it is important that we understand the limitations of, and uncertainties with, DNA-based methods. Natural England staff need to be able to understand how DNA-derived data can be used, and the confidence they can have in data derived using different DNA-based methods which have been validated to different stages.Natural England previously commissioned the development of a framework to enable end users to assess confidence in metabarcoding assays and results (Pagaling and others, under review; a copy of the framework can be provided by Natural England upon request by emailing monitoring.innovation@naturalengland.org.uk). By using the checklist and levelled scale, end users can appropriately interpret results. It also provides validation and reporting standards for the development of new metabarcoding assays. The framework will enable end-users to determine the recommended scenarios for application of a given assay and improve assay performance with further validation.This project will advance the metabarcoding assay validation framework by expanding upon previous work to conduct a further literature review and meta-analysis, covering more assays being used for different purposes and in different contexts. In addition beta testing of the framework and user guide will be required, as well as the creation and delivery of training materials and course .

Full notice text

Scope

Procurement description

DNA-based methods have the potential to significantly change how we monitor and assess ecosystems. Natural England has been exploring the use of these methods for environmental monitoring for several years, delivering a series of reports which focus on the development of DNA-based methods with potential in a particular area. These methods are now being used more widely within Natural England, particularly the detection of ecological communities using environmental DNA (eDNA). This is often conducted by employing metabarcoding assays which target specific species assemblages (e.g. fish, mammals) or broader taxonomic groups (e.g. vertebrates, invertebrates, bacteria).

The DNA Team aims to operationalise DNA-based methods and produces guidance for using them in monitoring projects. In order to provide sound recommendations, it is important that we understand the limitations of, and uncertainties with, DNA-based methods. Natural England staff need to be able to understand how DNA-derived data can be used, and the confidence they can have in data derived using different DNA-based methods which have been validated to different stages.

Natural England previously commissioned the development of a framework to enable end users to assess confidence in metabarcoding assays and results (Pagaling and others, under review; a copy of the framework can be provided by Natural England upon request by emailing monitoring.innovation@naturalengland.org.uk). By using the checklist and levelled scale, end users can appropriately interpret results. It also provides validation and reporting standards for the development of new metabarcoding assays. The framework will enable end-users to determine the recommended scenarios for application of a given assay and improve assay performance with further validation.

This project will advance the metabarcoding assay validation framework by expanding upon previous work to conduct a further literature review and meta-analysis, covering more assays being used for different purposes and in different contexts. In addition beta testing of the framework and user guide will be required, as well as the creation and delivery of training materials and course .

Main category

Services

Delivery regions

  • UK - United Kingdom

Total value (estimated, excluding VAT)

41666 GBP to 41666GBP

Contract dates (estimated)

11 September 2026, 00:00AM to 25 February 2027, 23:59PM

Contracting authority

Natural England

Identification register:

  • GB-PPON

Address 1: Seacole Building, 2 Marsham Street

Town/City: London

Postcode: SW1P 4DF

Country: United Kingdom

Public Procurement Organisation Number: PJDG-6588-XDMM

NUTS code: UKI32

Email: monitoring.innovation@naturalengland.org.uk

Organisation type: Public authority - central government

Procedure

Procedure type

Below threshold - open competition

Is the total value above threshold?

Below threshold

Lots

Divided into 1 lots

Lot number: 1

CPV classifications

  • 73000000 - Research and development services and related consultancy services
  • 80540000 - Environmental training services

Delivery regions

  • UK - United Kingdom

Lot value (estimated)

41666 GBP Excluding VAT

50000 GBP Including VAT

Sustainability

Small and medium-sized enterprises (SME)

Contract start date (estimated)

11 September 2026, 00:00AM

Contract end date (estimated)

25 February 2027, 23:59PM

Simple description of award criteria

Full details are available in the RFQ

Submission

Tender submission deadline

17 August 2026, 09:00AM

Enquiry deadline

10 August 2026, 14:00PM

Submission address and any special instructions

To apply for this opportunity you must submit your quotation meeting the requirements detailed in the Request for Quotation (RFQ) attached.Your response must be sent to monitoring.innovation@naturalengland.org.uk by 9:00 on 17/8/26If you have any clarification questions linked to this opportunity or the procurement process please submit these via email to monitoring.innovation@naturalengland.org.uk. Please note that, unless commercially sensitive, both the question and the response will be circulated to all tenderers.

May tenders be submitted electronically?

No

Documents

ID

A-19673

Document type

Bidding documents

Description

Commercial Response Template

ID

A-19705

Document type

Bidding documents

Description

Order form

ID

A-19734

Document type

Bidding documents

Description

RFQ

Coding

Commodity categories

ID Title Parent category
80540000 Environmental training services Training services
73000000 Research and development services and related consultancy services Research and Development

Delivery locations

ID Description
100 UK - All

Alert region restrictions

The buyer has restricted the alert for this notice to suppliers based in the following regions.

ID Description
There are no alert restrictions for this notice.

About the buyer

Main contact:
N/a
Admin contact:
N/a
Technical contact:
N/a
Other contact:
N/a

Further information

Date Details
No further information has been uploaded.

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